Phase Separation in Multicomponent Aqueous-Protein Solutions

Phase Separation in Multicomponent Aqueous-Protein Solutions

published in Advance ACS Abstracts, December 1, 1994. 0022-3654/95/2099-0454$09.00/0 we present here the results of experimental and theoretical studies of a ternary solution constituted of the proteins yma (designated as protein A), a “high-Tc” protein, and yw (designated as protein B), a “low-Tc” protein, in aqueous solution. The solution pH was kept constant at 7.1 by a 100 mM phosphate buff...

Monte Carlo study of phase separation in aqueous protein solutions

The binary liquid phase separation of aqueous solutions of g-crystallins is utilized to gain insight into the microscopic interactions between these proteins. The interactions are modeled by a square-well potential with reduced range l and depth e . A comparison is made between the experimentally determined phase diagram and the results of a modified Monte Carlo procedure which combines simulat...

Multicomponent Concentrated Aqueous Electrolyte Solutions

Phase separation during freezing upon warming of aqueous solutions.

Using differential scanning calorimetry, we show that the addition of solute(s) to emulsified water lowers the freezing temperature to <231 K, the homogeneous nucleation temperature of pure bulk water, or even completely suppresses freezing. In the latter case, freezing upon warming occurs above T(X) ≈ 150 K and leads to a phase separation into pure ice and a freeze-concentrated solution (FCS) ...

Aqueous two-phase systems for protein separation: phase separation and applications.

Aqueous two-phase systems (ATPS) that are formed by mixing a polymer (usually polyethylene glycol, PEG) and a salt (e.g. phosphate, sulphate or citrate) or two polymers, and water can be effectively used for the separation and purification of proteins. The partitioning between both phases is dependent on the surface properties of the proteins and on the composition of the two phase system as ha...